A new simple, accurate, precise and reproducible reverse phase liquid chromatographic method developed and validated for the quantification of diosmin in pharmaceutical dosage form. Separation was achieved under optimized chromatographic condition on inertsil C18 column (250× 4.6 mm, 5 µm), the mobile phase consisting of methanol: water (60:40 v/v). An isocratic elution was achieved at a flow rate 1.0 ml/min at an ambient temperature. The detection was carried out at 255 nm. The calibration curve was linear in concentration range 5-25 μg/ml (r2 0.999) for diosmin. The recovery studies were performed and the percentage recoveries were found to be 98.36% diosmin. So, proposed method was found to be simple, accurate, specific, linear and rugged.
Loading....